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RNA interference (RNAi) is a biological process in which RNA molecules inhibit gene expression, typically by causing the destruction of specific mRNA molecules. Historically, it was known by other names, including co-suppression, post transcriptional gene silencing (PTGS), and quelling. Only after these apparently unrelated processes were fully understood did it become clear that they all described the RNAi phenomenon. Andrew Fire and Craig C. Mello shared the 2006 Nobel Prize in Physiology or Medicine for their work on RNA interference in the nematode worm Caenorhabditis elegans, which they published in 1998.
Two types of small ribonucleic acid (RNA) molecules – microRNA (miRNA) and small interfering RNA (siRNA) – are central to RNA interference. RNAs are the direct products of genes, and these small RNAs can bind to other specific messenger RNA (mRNA) molecules and either increase or decrease their activity, for example by preventing an mRNA from producing a protein. RNA interference has an important role in defending cells against parasitic nucleotide sequences – viruses and transposons. It also influences development.
The RNAi pathway is found in many eukaryotes including animals and is initiated by the enzyme Dicer, which cleaves long double-stranded RNA (dsRNA) molecules into short double stranded fragments of ~20 nucleotide siRNAs. Each siRNA is unwound into two single-stranded (ss) ssRNAs, respectively the passenger strand and the guide strand. The passenger strand is degraded and the guide strand is incorporated into the RNA-induced silencing complex (RISC). The most well-studied outcome is post-transcriptional gene silencing, which occurs when the guide strand pairs with a complementary sequence in a messenger RNA molecule and induces cleavage by Argonaute, the catalytic component of the RISC complex. In some organisms, this process spreads systemically, despite the initially limited molar concentrations of siRNA.
RNAi is a valuable research tool, both in cell culture and in living organisms, because synthetic dsRNA introduced into cells can selectively and robustly induce suppression of specific genes of interest. RNAi may be used for large-scale screens that systematically shut down each gene in the cell, which can help to identify the components necessary for a particular cellular process or an event such as cell division. The pathway is also used as a practical tool in biotechnology, medicine and insecticides
RNA editing in viruses (i.e., measles, mumps, or parainfluenza) are used for stability and generation of protein variants. Viral RNAs are transcribed by a virus-encoded RNA-dependent RNA polymerase, which is prone to pausing and “stuttering” at certain nucleotide combinations. In addition, up to several hundred non-templated As are added by the polymerase at the 3’ end of nascent mRNA. These As help stabilize the mRNA. Furthermore, the pausing and stuttering of the RNA polymerase allows the incorporation of one or two Gs or As upstream of the translational codon. The addition of the non-templated nucleotides shifts the reading frame, which generates a different protein
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MEDICAL ROBOT KILL EBOLA VIRUS WITH UV LIGHT:
Medical robot kills Ebola with UV light
The company made the announcement as the ongoing Ebola epidemic continues to grab headlines around the world. To date, more than 7,470 people have become infected, nearly all of whom in West Africa, and at least 3,439 people have died. Yet these number are widely regarded as severe underestimates. In addition, the number of people infected with the disease is doubling every 20 to 30 days.
Ebola is a viral disease spread by close contact with bodily fluids such as blood, vomit or diarrhea. Between 60 and 90 percent of infections end with death.
Robot sterilizes rooms remotelyAccording to Xenex, Little Moe destroys the DNA of viruses using a variety of ultraviolet radiation known as UV-C. Along with the more familiar UV-A and UV-B rays which promote vitamin D synthesis and can lead to sunburn in excessive amounts, UV-C rays are also emitted by the sun. But because the Earth's ozone layer prevents UV-C rays from reaching the surface, organisms have not evolved resistance to them as they have to UV-A and UV-B.
Little Moe is an automated robot with a 62-inch, extendable neck. The neck contains a special bulb that fires 1.5 UV-C pulses per second in all directions, delinking and fusing the DNA of microorganisms. Because UV-C rays can damage the human eye, the robot is designed to be placed in an empty room and operated remotely. According to Xenex, the UV rays cannot pass through walls, windows or hospital safety glass.
Little Moe is just one of a myriad of measures now being suggested to help stem the uncontrolled spread of Ebola. Most such efforts, however, have focused on pharmaceutical drugs rather than medical devices.
Drug companies are scrambling to develop a vaccine for the disease and have turned to analyzing the blood of people who have recovered from Ebola infections. Indeed, blood transfusions from survivors appear to improve the recovery chances of current Ebola patients, suggesting that perhaps antibodies against the disease can be transferred from one person to another. British Ebola survivor William Pooley has recently traveled to the United States to donate his blood for vaccine research.
Drugs also being testedA number of different companies are also testing out experimental drugs to treat people who have already contracted Ebola. The most famous of these, ZMapp, has already been used experimentally to treat seven people, five of whom later recovered. This use liquidated all existing supplies of the drug, however, and the company has announced that it will take months to make more. Yet the drug has also shown promise in one animal study, published in the journal Nature on August 29: Of 18 rhesus monkeys infected with the virus and given ZMapp within five days, 100 percent recovered.
Dallas Ebola patient Thomas Duncan is being treated with another experimental drug, named brincidofovir. Unlike ZMapp, which consists of a cocktail of antibodies, brincidofovir is an anti-retroviral drug, much like HIV medications.
Other experimental Ebola drugs include the flu drug favipiravir (T-705) and the drug BCX4430. Canadian company Tekmira was testing another drug, but those trials have been halted due to safety concerns.
Doctors emphasize that no drug has yet been proven effective against Ebola. In contrast, proper supportive care such as hydration, saline solution and oxygen treatment has been proven to improve survival rates.
Learn all these details and more at the FREE online Pandemic Preparedness course at www.BioDefense.com
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